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. 2016 Nov 3;6:36264. doi: 10.1038/srep36264

Figure 1. The MTR variants rs28372871 T > G and rs1131450 G > A down-regulate MTR expression at the levels of transcription and translation, respectively.

Figure 1

(A) Luciferase expression is significantly decreased in the minor G allele construct compared with the major T construct in different cell types (47.2% reduction in LNCaP cells and 51.4% reduction in PC3 cells). (B) Quantitative real-time polymerase chain reaction (PCR) analysis of MTR in vivo mRNA expression in 52 human prostate tissue samples with different rs28372871 T > G genotypes. All values have been normalized to the level of GAPDH. (C) In luciferase assays, a plasmid construct with the minor A allele manifested luciferase activity significantly reduced by 31.1% compared with the major G allele in LNCaP cells. This value was 26.0% in PC3 cells. (D) Quantitative real-time polymerase chain reaction analysis of MTR in vivo mRNA expression in 52 human prostate tissue samples with different rs1131450 G > A genotypes. All values have been normalized to the level of GAPDH. Each value represents the mean ± SD of three independent experiments, and each experiment was performed in triplicate. ***indicates P < 0.001.