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. 2016 Nov 3;6:36264. doi: 10.1038/srep36264

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Knockdown efficiency of gradient MTR siRNA was measured using quantitative real-time polymerase chain reaction. (B) Cellular homocysteine concentration after transfection with gradient MTR siRNA. (C) Cellular methionine level after transfected with gradient MTR siRNA. (D) Cellular SAM level after transfection with gradient MTR siRNA. (E) Cellular SAH level after transfection with gradient MTR siRNA. (F) Ratio of SAH/SAM after transfection with gradient MTR siRNA. Each value represents the mean ± SD of three independent experiments, and each experiment was performed in triplicate.