Figure 4. Venn-regional analysis of gut or liver advantage transcripts.

(A) Venn analysis among data of 0 h, 12 h, and 36 h, for both the total and immune differential transcripts between gut and liver, and the percentage of immune transcripts vs total transcripts was labeled in each region. The total No. of differentially expressed genes between gut and liver was 5788, 4702 and 5952 at 0 h, 12 h and 36 h respectively, and after filtered by the immune gene library the numbers decreased to 927, 872, and 942. And the dashed red lined regions, including region a, b, e and d, indicated data at 0 h, in another word in homeostasis status, whereas the dashed green lined regions (f, c and g) specifically indicated data upon inflammational states (12 h and 36 h). (B) Transcripts No. involved in major immune processes for each region. For gut advantage transcripts, in regions a, b and c, most of them were involved in “pattern recognition”, “T/B cell activation”, “other genes related to immune response”, “inflammatory cytokines and receptors”, as well as “antigen processing and regulators”; in regions d and e, except for the above mentioned, most of them were also involved in “adapters, effectors and signal transducers”; in regions f and g, most of them were involved in “pattern recognition”, “inflammatory cytokines and receptors”, “T/B cell antigen activation”, as well as “other genes related to immune response”. On the other hand, for liver advantage ones, in region a, most were involved in “other genes related to immune response”, “complement system”, “pattern recognition”, as well as “acute phase reactions”. In regions d and g, most were involved in “other genes related to immune response”, “inflammatory cytokines and receptors”, as well as “pattern recognition”, while in region f, also in “antigen processing and regulators”, as well as “T/B cell activation”, except for the above mentioned. In other regions (b, c and e), the liver advantage genes were much fewer.