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. 2016 Nov 3;6:36433. doi: 10.1038/srep36433

Figure 2. The whole primary sequence of miR-15a-16 was assembled by multiple datasets and SMART cloning sequencing.

Figure 2

Integrated analysis of a long noncoding RNA-seq dataset (GSM694305) in muscle, another long noncoding RNA-seq dataset (GSE28080) in muscle and transcriptome data (SRP038897) using long-read sequencing technology resulted in 9 contigs mapped to the flanking of miR-15a-16 cluster. Then, using 5′ rapid amplification of cDNA ends (RACE) technology, a 1028-bp transcript was identified to overlap 6 contigs from high-throughput sequencing, resulting in a transcript isoform fragment of about 5-kb in length.