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. 2016 Nov 3;6:36346. doi: 10.1038/srep36346

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Copyright © 2016, The Author(s)

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Black circles correspond to Hk4-Hk45 with native sequences. The K₁₂ values for Hk14VA, Hk14LA and Hk14VALA mutants were adopted from the previous study²⁷. Numbers 1 and 2 refer to two ranges of stability increase as discussed in the text.