. 2016 Nov 3;6:36346. doi: 10.1038/srep36346

Table 1. Apparent formation constants (logK ₁₂) of Zn(Hk)₂ complexes of zinc hook peptides and their mutants at pH 7.4, I = 0.1 M, 25 °C.

Hook peptide	logK₁₂	Mutant peptide	logK₁₂
Hk4	14.93 ± 0.02	Hk4VA	14.41 ± 0.02
Hk5	15.37 ± 0.02	Hk4PA	14.19 ± 0.02
Hk6	16.44 ± 0.02	Hk4PAVA	13.96 ± 0.02
Hk8	17.78 ± 0.01	Hk45LA	19.06 ± 0.08
Hk10	18.64 ± 0.01	Hk45VA	19.78 ± 0.08
Hk12	19.02 ± 0.01	Hk45LAVA	17.89 ± 0.08
Hk14	19.19 ± 0.01
Hk23	19.49 ± 0.04	Depsipeptide	logK₁₂
Hk27	19.77 ± 0.04	depsiHk8	15.33 ± 0.01
Hk31	20.47 ± 0.09	depsiHk14	16.21 ± 0.01
Hk37	20.69 ± 0.06	depsiHk14LA	14.73 ± 0.03
Hk45	20.74 ± 0.06	depsiHk14VALA	13.97 ± 0.02

Values were calculated from cumulative protonation and stability constants determined potentiometrically (Tables S1 and S6) or determined spectropolarimetrically in competition experiments with zinc chelators (Fig. S4) and are not the results of ITC data fits.

Table 1. Apparent formation constants (logK 12) of Zn(Hk)2 complexes of zinc hook peptides and their mutants at pH 7.4, I = 0.1 M, 25 °C.

Table 1. Apparent formation constants (logK ₁₂) of Zn(Hk)₂ complexes of zinc hook peptides and their mutants at pH 7.4, I = 0.1 M, 25 °C.