Table 2. Thermodynamic parameters of Zn(Hk)₂ complex formation at pH 7.4, I = 0.1 M, 25 °C.

Zinc hook peptide	ΔG° (kcal/mol)	ΔHITC (kcal/mol)	ΔH° (kcal/mol)	ΔH°Zn-pep (kcal/mol)	nHΔH°CysH (kcal/mol)	−TΔS° (kcal/mol)
Hk4	−20.37 ± 0.03	−12.1 ± 0.03	6.54	−25.08	31.62	−26.92
Hk5	−20.97 ± 0.03	−13.5 ± 0.02	4.75	−26.19	30.94	−25.73
Hk6	−22.43 ± 0.03	−15.5 ± 0.02	2.15	−27.77	29.92	−24.58
Hk8	−24.26 ± 0.01	−19.3 ± 0.02	−1.85	−31.43	29.58	−22.41
Hk10	−25.43 ± 0.01	−18.2 ± 0.02	−2.16	−29.36	27.2	−23.28
Hk12	−25.95 ± 0.01	−18.3 ± 0.02	−2.26	−29.46	27.2	−23.70
Hk14	−26.18 ± 0.01	−18.2 ± 0.02	−2.36	−29.22	26.86	−23.82
Hk23	−26.59 ± 0.05	−17.7 ± 0.03	−2.17	−28.52	26.35	−24.43
Hk27	−26.97 ± 0.05	−18.1 ± 0.03	−2.57	−28.92	26.35	−24.41
Hk31	−27.9 ± 0.01	−19.5 ± 0.03	−3.88	−30.40	26.52	−24.03
Hk37	−28.22 ± 0.08	−20.6 ± 0.04	−4.88	−31.57	26.69	−23.35
Hk45	−28.29 ± 0.08	−22.5 ± 0.03	−6.87	−33.39	26.52	−21.43

The ΔG° values were calculated based on apparent formation constants K₁₂ presented in Table 1 using equation (7).