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. 2016 Nov 3;6:36323. doi: 10.1038/srep36323

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Viability of control trophozoites and NAT cultivated in presence of GSNO (110 μM). Control trophozoites and NAT. The number of living trophozoites was determined after 24 h and 48 h of culture. Data are expressed as the mean ± standard deviation of three independent experiments that were repeated twice. *p ≤ 0.05 and is the significance of the difference between the viability of the control trophozoites and NAT after 48 h of culture according to the results of an unpaired Student’s t-test. (B) Viability of control and GSNO trophozoites exposed to CysNO (500 μM). Data are expressed as the mean ± SD of three independent experiments that were repeated twice. *p ≤ 0.05 and is the significance of the difference between the viability of the control trophozoites and NAT according to the results of an unpaired Student’s t-test. (C) Viability of NAT exposed to metronidazole or to H₂O₂. Data are expressed as the mean ± SD of three independent experiments that were repeated twice. *p ≤ 0.05 and is the significance of the difference between the viability of the control trophozoites and NAT according to the results of an unpaired Student’s t-test. (D) Viability of control E. histolytica trophozoites and NO-adapted trophozoites (NAT) after exposure to activated macrophages. Data are expressed as the mean ± SD of three independent experiments that were repeated twice. *p ≤ 0.05 and is the significance of the difference between the viability of the control trophozoites and NAT according to the results of an unpaired Student’s t-test. (E) Adhesion of E. histolytica control trophozoites and NO-adapted trophozoites (NAT) to HeLa cells. The amount of adhesion of the control trophozoites was set at 100%. Data are expressed as the mean ± SD of three independent experiments that were performed in triplicate. The adhesion of the control trophozoites was significantly different (p ≤ 0.05) to the adhesion of trophozoites that were exposed to GSNO according to the results of an unpaired Student’s t-test. No significant difference between the adhesion of control trophozoites and the NAT was detected.