Figure 3. NAOD expression in NAT, NAOD trophozoites and mNAOD trophozoites.

(A) qRT-PCR was used to monitor naod expression using cDNA from NAT. The QRT-PCR values were normalized to the level of rDNA⁷⁴. Data are expressed as the mean ± SD of three independent experiments. *p ≤ 0.05 and is the significance of the difference between the expression levels of naod in the control trophozoites and NAT according to the results of an unpaired Student’s t-test. (B) Western blot analysis was performed on total protein extracts that were prepared from control trophozoites and NAT. The proteins were separated on 12% SDS-PAGE gels and analyzed by western blotting with a NAOD antibody or actin antibody. The figure displays a representative result from three independent experiments. (C) qRT-PCR was used to monitor naod expression using cDNA from pcontrol trophozoites, NAOD trophozoites and mNAOD trophozoites. The QRT-PCR values were normalized to the level of naod expression in pcontrol trophozoites. Data are expressed as the mean ± SD of three independent experiments. *p ≤ 0.05 and is the significance of the difference between the expression levels of NAOD in the pcontrol trophozoites, NAOD trophozoites and mNAOD trophozoites according to the results of an unpaired Student’s t-test. (D) Western blot analysis was performed on total protein extracts that were prepared from pcontrol trophozoites, NAOD trophozoites and mNAOD trophozoites. The proteins were separated on 12% SDS-PAGE gels and analyzed by western blotting with an HA antibody or actin antibody. Cropped image of representative results from three independent experiments. Full length image is presented in Supporting Information S4.