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. 2016 Nov 3;6:36323. doi: 10.1038/srep36323

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Copyright © 2016, The Author(s)

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(A) Analysis of eluted His-tagged NAOD, His-tagged mNAOD and His-tagged His-tagged E. coli NAOD (ArgE) was determined by SDS-PAGE and Coomassie staining (A cropped image is showed. Full length blots are presented in Supporting Information S4). Lane 1, His-tagged mNAOD, Lane 2 His-tagged NAOD, Lane 3 His-tagged E. coli NAOD (ArgE). (B) Specific activity of His-tagged mNAOD, His-tagged NAOD, and His-tagged E. coli NAOD (ArgE). One unit (U) corresponds to 1 nmol ornithine produced/in/mg protein.