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. 2016 Nov 3;6:35677. doi: 10.1038/srep35677

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Copyright © 2016, The Author(s)

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(a) Signal of antisense HaEXPB2 DIG-labeled cDNA probes localized within the subventral glands (SVGs). (b) Sense probes as a negative control. Scale bar = 20 μm. (c) Developmental expression pattern of HaEXPB2. The relative expression level of HaEXPB2 was quantified using qPCR for six different H. avenae stages. The fold change values were calculated using the 2-ΔΔCt method and presented as the change in mRNA level in various nematode developmental stages relative to that of egg. Each column represents the mean of three independent assays with standard deviation. J2: pre-parasitic second-stage juvenile; parJ2, J3 and J4: parasitic second-, third- and fourth-stage juvenile, respectively.