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. 2016 Nov 3;6:35677. doi: 10.1038/srep35677

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The purified protein MBP, MBP: HaEXPB2, MBP: HaEXPB2ΔCBD were mixed with cotton linters separately. The mixture of protein and substrate was centrifuged to obtain supernatant (S) and pellet (P) fraction. Input (I) indicates the mixture of protein and substrate before separation. The presence of the HaEXPB2 with CBD is required for the protein to bind to cellulose.