Fig. 5.

Treatment with plerixafor does not alter intratibial tumor growth when administered subsequent to tumor formation. a Diagram of experiment timeline. On Day 0, PC-3 M-luc2 cells were injected intratibially. After allowing tumors to grow for 18 days, saline control or plerixafor treatment was started via an osmotic pump. b After 21, days post-injection of tumor cells, in vivo luciferase imaging was performed. c Tumor growth at 21 days post injection of cells was determined by in vivo luciferase imaging (n = 10). NS represents no significant difference between groups. d Ex vivo x-ray imaging of media injected (left) and tumor bearing (right) tibiae was performed at 26 days post-injection. e H&E was performed on tissue sections from PC-3 M-luc2 intratibial tumors treated with saline control or plerixafor. Representative images were taken at 5X and digitally merged. f Tissue sections from PC-3 M-luc2 intratibial tumors were immunostained with anti-CXCR4 antibody. g Proliferation of PC-3 M-luc2 tumors was analyzed by Ki67 staining; the average number of Ki67+ cells from five 20X fields was calculated