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. 2016 Nov 3;12:42. doi: 10.1186/s13007-016-0142-6

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Flowchart of optimized method for discovering mRNA-bound proteome from Arabidopsis leaf mesophyll protoplasts. Main steps listed in numbers from 1 to 10. Putative cellular and molecular processes illustrated by cartoons and photos. Details for each step described in “Methods” section