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. 2016 Nov 2;17:852. doi: 10.1186/s12864-016-3197-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Workflow of RGAugury. The pipeline was designed to use protein sequences to detect conserved domains and motifs found in genes involved in plant resistance and identify RGAs by integrating results generated from five programs: BLAST, InterProScan, pfam_scan, nCoil and Phobius. The annotated candidates for four different RGA types are exported as plain files. Analyses performed in parallel mode are labelled in blue. Intermediate results are indicated by a dashed-line box. GFF3: Generic Feature Format version 3; CC: coiled-coil; LRR: leucine-rich repeat; NB-ARC: nucleotide binding adapter shared by APAF-1, R gene products and CED-4; STTK: serine/threonine and tyrosine kinase; LysM: lysin motif; TM: transmembrane