Fig. 3.

Immunohistochemistry for doublecortin (DCX) in the dentate gyrus in vehicle-treated (vehicle, a, and b), garlic essential oil-treated (garlic oil, c, and d) and chives essential oil-treated (chives oil, e, and f) groups. DCX-immunoreactive (⁺) neuroblasts are seen in the subgranular zone of the dentate gyrus and their dendrites are observed in the molecular layer (ML) of the dentate gyrus. Note that DCX⁺ neuroblasts and their dendrites are abundant in the garlic essential oil-treated group, while in the chives essential oil-treated group, DCX⁺ neuroblasts and their dendrites are few. GCL, granule cell layer; ML, molecular layer; PL, polymorphic layer. Scale bar = 50 μm (a, c, e), 25 μm (b, d, f). g Number of DCX⁺ neuroblasts per section for each group (n = 5 per group; *p < 0.05, versus vehicle group). Data are presented as mean ± SEM