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. 2016 Nov 3;16:210. doi: 10.1186/s12872-016-0385-1

Fig. 2.

Fig. 2

Flow cytometric gating strategy of CD3-positive T-cells expressing nicotinic acetylcholine receptor (AchR). After exclusion of doublets (a), lymphocytes were characterized by anti-CD3 staining (b) and by back-gating in a FSC/SSC plot (c). Plots d and e are representative of isotype control and the corresponding sample staining positive for CD3 + AchR+