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. 2016 Nov 3;16:210. doi: 10.1186/s12872-016-0385-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Flow cytometric gating strategy of CD19-positive B-cells expressing nicotinic acetylcholine receptor (AchR). After exclusion of doublets (a), B-cells were characterized by anti-CD19 staining (b) and by back-gating in a FSC/SSC plot (c). Plots d and e are representative of isotype control and the corresponding sample staining positive for CD19 + AchR+