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Flow cytometric gating strategy of CD14-positive monocytes expressing nicotinic acetylcholine receptor (AchR). After exclusion of doublets (a), CD15++ granulocytes and CD19+ B-cells were excluded (b). Monocytes were characterized by anti-CD86- (c) and by anti-CD14-staining (d). Plots e and f are representative of isotype control and the corresponding sample staining positive for CD14 + AchR+
