Fig. 2.

A THP cells were cultured for 96 h in 10% RPMI 1640 containing PMA in the presence of cyclophilin A (100 ng/mL) in NG/HG conditions to induce differentiation. The differentiation of monocyte to macrophage was analysed by transmitted detector of confocal microscopy. THP-1 cells incubated in the presence of cyclophilin A and HG exhibited cell flattening and development of cytoplasmic processes. B Western blot analysis of scavenger receptor markers, CD 36 and LOX 1 of glucose activated monocyte differentiated macrophages after treatment with cyclophilin A (100 ng/mL). Cyclophilin A treated macrophages in high glucose conditions resulted in a significant increase in LOX-1 and CD36 protein expression. Protein densities of immunoreactive bands measured by the Quantity One 1D analysis software program. Data are presented as mean ± SD (n = 3) and asterisk represents p < 0.05