Fig. 7.

a Flow cytometric analysis of Cell Rox (5 µM) uptake by THP differentiated macrophages cultured in high glucose (20 mM/L) during foam cell formation. Monocyte differentiated macrophages were treated with cell ROX reagent at a final concentration of 5 µM. Cells treated with cyclophilin A had increased oxidized LDL induced cytosolic ROS production. The right panel shows fluorescence intensity analyzed by FACS using FACS Diva v8.0 software. Cells were quantitated by subtracting the cell autofluorescence of the treated samples and expressed as mean fluorescence intensity. b Thiobarbituric acid-reactive substances (TBARS) assay to assess the levels of lipid peroxides in medium of cultured THP1 cells treated with or without cyclophilin A. c Catalase enzyme activity assay of cells treated with oxLDL in the presence and absence of cyclophilin A. Data are presented as mean ± SD (n = 3) and asterisk represents p < 0.05