Table 3.
Effects of organic acids and phytase supplementation on histomorphology of duodenum of broilers at 42nd day
| Treatments |
Villus
height
(µm) |
Villus width ( µm) | Crypt depth (µm) | Epithelium thickness (µm) | Number of Goblet cells |
Villus
height
/
Crypt depth ratio |
|
|---|---|---|---|---|---|---|---|
| Vinegar (%) | 0.00 | 1361 | 139 | 143 | 199 | 867 | 9.51 |
| 2.00 | 1346 | 140 | 142 | 209 | 876 | 9.47 | |
| Citric acid (%) | 0.00 | 1337b | 136b | 145 | 199 | 868 | 9.22b |
| 2.00 | 1370a | 141a | 141 | 201 | 876 | 9.71a | |
| Phytase (FTU) | 0.00 | 1356 | 137b | 146a | 199 | 870 | 9.28b |
| 500 | 1351 | 141a | 140b | 201 | 875 | 9.65a | |
| Standard Error | 10.00 | 1.10 | 1.30 | 2.20 | 8.00 | 0.12 | |
|
p-value
| |||||||
| Vinegar | 0.31 | 0.38 | 0.57 | 0.72 | 0.51 | 0.54 | |
| Citric acid | 0.04 | 0.02 | 0.07 | 0.57 | 0.51 | 0.01 | |
| Phytase | 0.74 | 0.03 | 0.03 | 0.57 | 0.68 | 0.02 | |
| Vinegar × Citric | 0.56 | 0.08 | 0.39 | 0.87 | 0.64 | 0.90 | |
| Vinegar × Phytase | 0.38 | 0.75 | 0.08 | 0.06 | 0.96 | 0.06 | |
| Citric × Phytase | 0.25 | 0.08 | 0.04 | 0.64 | 0.07 | 0.00 | |
| Vinegar × Citric × Phytase | 0.06 | 0.31 | 0.51 | 0.08 | 0.40 | 0.06 | |
Different superscript letters within a column indicate significant differences at p ˂ 0 .05.