Table 1.
A comparison of physiological, behavioural and endocrine markers across vehicle and 667-COUMATE-treated mothers.
| Measure of interest | Vehicle | 667-COUMATE | Statistical significance |
|---|---|---|---|
| Litter measures | |||
| Initial litter size | 6.9 ± 0.4 pups | 6.6 ± 0.6 pups | t[29] = 0.48, p = 0.63 |
| Number of pups found dead per mother across course of experiment | 1.5 (95%CI: 1–2) | 1.0 (95%CI: 0–2) | p = 0.60 |
| Elevated plus maze measures | |||
| 100x (open arm time/closed + open arm time) | 25 ± 4 | 31 ± 4 | t[29] = −1.11, p = 0.28 |
| Latency to first open arm entry (s) | 9.7 (95%CI: 3.8–14.3) | 2.4 (95%CI: 2.1–4.5) | p = 0.021 |
| Closed arm entries | 24 ± 2 | 21 ± 2 | t[29] = 1.04, p = 0.31 |
| Rears | 0 (95%CI: 0–0) | 21 (95%CI: 0–81) | p = 0.040 |
| Head dips | 46 ± 9 | 35 ± 5 | t[20.4] = 1.09, p = 0.29 |
| Stretch-attend postures | 18.5 (95%CI: 9–61) | 14 (95%CI: 8–29) | p = 0.42 |
| Fecal boli | 0 (95%CI: 0–2) | 1 (95%CI: 0–2.5) | p = 0.38 |
| Maternal serum steroid hormone measurements | |||
| Dehydroepiandrosterone sulfate (DHEAS, ng/ml) | 4.4 (95%CI: 0.0–45.5) | 32.2 (95%CI: 10.7–43.1) | p = 0.07 (one-tailed) |
| Dehydroepiandrosterone (DHEA, ng/ml) |
0.059 (95%CI: 0.000-0.232) | 0.052 (95%CI: 0.000–0.119) | p = 0.45 (one-tailed) |
| Corticosterone (ng/ml) | 9.0 (95%CI: 5.5–13.8) | 10.0 (95%CI: 3.2–19.1) | p = 0.91 |