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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Brain Res. 2016 Feb 2;1636:62–73. doi: 10.1016/j.brainres.2016.01.045

Fig. 4.

Fig. 4

Electron micrographs of ENK-ir (particulate Vector-VIP reaction product) and NET-ir (dense, diffuse DAB reaction product) in the BLa. Small arrowheads indicate representative Vector-VIP particles in ENK+ structures. (A) In this field ENK-ir is found in various structures including a myelinated axon (ENK-Ax), spine head (ENK-Sp), and a small-caliber dendrite (ENK-SD) that gives rise to an unlabeled spine (Sp); an NET+ axon terminal (t-NET) containing a large dense core vesicle (white asterisk) contacts both this dendrite as well as its spine. The former contact appears to be a symmetrical synapse (arrow) while the latter was judged to be an apposition (black asterisk). An unlabeled terminal (t-U) is also in the field. (B) An NET+ axon terminal (t-NET) forms an apposition (asterisk) with a small-caliber dendrite (SD) that is very lightly labeled for ENK. This field also contains an unlabeled axon terminal (t-U) that forms an asymmetrical synapse (arrow) with an ENK+ spine (ENK-Sp). Two ENK+ axon terminals are seen in the lower right corner (t-ENK); the upper terminal forms an asymmetrical synapse (arrow) with an unlabeled spine. Scale bars = 0.5 µm.