Fibroblasts modulate the response of PDA cells to gemcitabine in vitro.
(A) Proliferation of GFP-AsPC1 cells in co-culture with NHF544 or cultured alone. Green fluorescence in GFP-AsPC1 cells was measured at 485-nm excitation and 538-nm emission. Bars represent average relative fluorescence units of triplicates ± SE. *P < .05.
Proliferation of CAFs (CCK-8 assay) cultured for 48 hours on collagen-coated or uncoated plates (B, P = .01) and on native or TG2-mediated cross-linked collagen (C, P < .001). Bars represent average measurements ± SE.
(D) Relative number of alive GFP-AsPC1 cells cultured alone or with CAFs after 48 hours of gemcitabine treatment. Bars represent average measurements ± SE. *P < .05.