Table 2. SAXS analysis of full-length TcCRT and its crystallized construct: parameters derived from the data collected at different concentrations (in mg ml−1) and from the merged scattering curve.
| Full-length TcCRT | Crystallized TcCRT construct | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Curve | Merged | 1.63 | 3.09 | 7.05 | 10.40 | Merged | 1.80 | 4.73 | 5.83 | 11.15 |
| I(0)† | 42.8 ± 0.05 | 42.9 ± 0.07 | 42.8 ± 0.04 | 38.7 ± 0.02 | 34.5 ± 0.03 | 24.0 ± 0.01 | 24.8 ± 0.03 | 24.5 ± 0.01 | 24.6 ± 0.01 | 24.79 ± 0.01 |
| Linear segment† | 12–44 | 24–61 | 11–54 | 12–65 | 12–47 | 22–119 | 28–101 | 20–173 | 22–132 | 16–93 |
| R g † (nm) | 3.74 ± 0.01 | 3.79 ± 0.01 | 3.73 ± 0.01 | 3.37 ± 0.03 | 3.10 ± 0.01 | 2.17 ± 0.01 | 2.17 ± 0.01 | 2.11 ± 0.001 | 2.10 ± 0.001 | 1.98 ± 0.06 |
| R g ‡ (nm) | 3.86 | 3.92 | 3.82 | 3.58 | 2.88 | 2.12 | 2.18 | 2.12 | 2.11 | 2.05 |
| D max ‡ (nm) | 13.09 | 13.11 | 12.78 | 11.55 | 17.96 | 7.38 | 7.43 | 7.06 | 7.34 | 6.62 |
| Porod volume‡ (nm3) | 63.91 | 90.43 | 65.72 | 77.29 | 74.44 | 52.64 | 57.19 | 55.44 | 51.45 | 48.23 |
Guinier analysis using PRIMUS.
Indirect Fourier transform analysis using GNOM.