Skip to main content
. 2016 Sep 14;3(Pt 6):408–419. doi: 10.1107/S2052252516012847

Table 2. SAXS analysis of full-length TcCRT and its crystallized construct: parameters derived from the data collected at different concentrations (in mg ml−1) and from the merged scattering curve.

  Full-length TcCRT Crystallized TcCRT construct
Curve Merged 1.63 3.09 7.05 10.40 Merged 1.80 4.73 5.83 11.15
I(0) 42.8 ± 0.05 42.9 ± 0.07 42.8 ± 0.04 38.7 ± 0.02 34.5 ± 0.03 24.0 ± 0.01 24.8 ± 0.03 24.5 ± 0.01 24.6 ± 0.01 24.79 ± 0.01
Linear segment 12–44 24–61 11–54 12–65 12–47 22–119 28–101 20–173 22–132 16–93
R g (nm) 3.74 ± 0.01 3.79 ± 0.01 3.73 ± 0.01 3.37 ± 0.03 3.10 ± 0.01 2.17 ± 0.01 2.17 ± 0.01 2.11 ± 0.001 2.10 ± 0.001 1.98 ± 0.06
R g (nm) 3.86 3.92 3.82 3.58 2.88 2.12 2.18 2.12 2.11 2.05
D max (nm) 13.09 13.11 12.78 11.55 17.96 7.38 7.43 7.06 7.34 6.62
Porod volume (nm3) 63.91 90.43 65.72 77.29 74.44 52.64 57.19 55.44 51.45 48.23

Guinier analysis using PRIMUS.

Indirect Fourier transform analysis using GNOM.