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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Nature. 2016 Mar 21;532(7597):103–106. doi: 10.1038/nature17156

Extended Data Figure 8. Locations of infected neurons after injection of AAV DIO-hM4Di-mCherry.

Extended Data Figure 8

AAV DIO-hM4Di-mCherry was injected into the AmPir (a, b) or PLCo/PMCo (c) and brain sections immunostained for mCherry to detect infected neurons. Shown are schematic sections from a mouse brain atlas30 that correspond to immunostained sections. The location of AmPir is indicated in purple (a–c), and the location of PLCo/PMCo is indicated in green (c). Regions that contained the nuclei of mCherry+ neurons are indicated in red in each section. Schematics are shown for 6 animals per area (per condition) injected with CNO and then exposed to bobcat urine (a, c) or TMT (b) to test the effects of silencing the AmPir or PLCo/PMCo.