Skip to main content
. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Nature. 2016 Mar 21;532(7597):103–106. doi: 10.1038/nature17156

Figure 3. Chemogenetic activation of AmPir induces stress hormone increase.

Figure 3

a–d, mCherry immunostaining (red) after injection of AAV DIO-hM3Dq-mCherry (a) into the AmPir (b), pPir (c), or MEA (d). Scale bars, 2 mm (left), 400 μm (right).

e, Plasma ACTH concentration following injection of CNO or saline into mice expressing hM3Dq in pPir, AmPir, or MEA. (n=5–6 per condition. Error bars indicate SEM. *p<0.05. Mann-Whitney U test.)

f, PVN neurons colabeled for CRH (red) and nuclear c-Fos (ncFos) (green) after injection of saline or CNO into mice expressing hM3Dq in AmPir. Scale bar, 20 μm

g, Percentage of PVN CRH+ neurons colabeled for nc-Fos in mice treated as in (e). (n=4–6 per condition. Error bars indicate SEM. *p<0.05. Mann-Whitney U test.)

h, AmPir neurons labeled for nArc (green) (arrowheads) following saline or CNO injection of mice expressing hM3Dq in AmPir. Scale bar, 50 μm.

i, Number of nArc+ neurons in AmPir, pPir, or MEA in mice treated as in (e). (n=4 per condition. Error bars indicate SEM. *p<0.05, Unpaired t-test.)