Fig. 2. Ocean cells, but not Island cells, exhibit context-specific Ca²⁺ activity.

(A) Experimental schedule showing sequential exposure to two contexts (each exposure is 5 min): A → B → A → B. (B and C) Distribution of different types of cell responses in MEC of an Ocean mouse (B) and an Island mouse (C) observed through microendoscopy during exposures to both contexts. Response to Context A (red cells), Context B (green cells), both contexts (yellow cells), or neither context (blue cells). Cell responses were found by picking small regions of interest (ROIs) (~ 1/3 of cell body size) at the center of the cell bodies. (D) Example of Context A-specific Ca²⁺ activity in a cell from an Ocean mouse that explored both Context A and B. (E–H) Ca²⁺ activity in an Ocean mouse that explored both contexts. Four examples are shown for each different type of Ocean cell response: Context A-specific Ca²⁺ activity (E), Context B-specific Ca²⁺ activity (F), Ca²⁺ activity in both contexts (G), and Ca²⁺ activity in neither context (H). (I–J) Ca²⁺ activity in an Island mouse that explored both contexts: 4 example Island cells that were active in both contexts (I), and 4 example Island cells that were active in neither context (J).