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. 2016 Jun 28;16(15-16):2246–2256. doi: 10.1002/pmic.201500488

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© 2016 The Authors. PROTEOMICS published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Isolated effect of iRT precision on identification. (A) Imprecise iRT values were simulated by adding normally distributed iRT noise to the reference spectral library (0, 1, 2, 4, 8 and 16 iRT SD). The modified spectral libraries were used for the targeted analysis of a HeLa in a technical triplicate DIA. Additionally, the DIA runs were analyzed using no RT prediction (Full XIC) and linear regression based on the 11 original iRT peptides. The peptide (B) and protein (C) identifications were calculated for the analyses with the modified spectral libraries. The mean identifications with SD were plotted.