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. 2016 Jun 28;16(15-16):2246–2256. doi: 10.1002/pmic.201500488

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© 2016 The Authors. PROTEOMICS published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Experimental factors influencing iRT precision. (A) Experimental iRT values were determined for nine practically relevant experimental factors: stationary and mobile phases, column and gradient length, column temperature, flow rates, column replicates, sample types and sample on column amount. The SD of the iRT values for overlapping peptides of the most diverging conditions per factor were calculated. (B) The deviation in iRT to the reference spectral library was determined for all conditions within the experimental factors.