Figure 2.

KDM4C inhibition with NCDM-32b affects histone demethylation levels. (A) Immunofluorescence of HCC38, MDA-MB-436, and MDA-MB-453 cell lines treated (right) or untreated (left) with inhibitor. Note the significant increment in fluorescence intensity for treated samples with respect to the untreated cells. All images were recorded at 80 milliseconds under the same conditions of exposition. White scale bar: 10 μm. (B) Quantification of histone 3 lysine 9 trimethyl levels by ImageJ software (National Institutes of Health), showing a significant increment on H3K9-3me levels up to six times with respect to the control for all treatments.