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. 2016 Nov 3;11(11):e0166010. doi: 10.1371/journal.pone.0166010

Fig 5. Melatonin suppresses the increase in TLR4 immunofluorescence in microglia in neonatal rats after hypoxic treatment.

Fig 5

Confocal images showing TLR4 expression in lectin labeled (green) microglia in the corpus callosum of control, hypoxia and hypoxia+melatonin rats at 3 days after the hypoxic exposure. Increase in TLR4 expression in microglia is evident in hypoxic rats. Note TLR4 immunofluorescence intensity is attenuated in hypoxia+melatonin rats compared with that in the hypoxic rats. The lower graph showing the optical density of TLR4 on microglia of different groups. Note the optical density of TLR4 on microglia was increased in rats following hypoxia challenge and the optical density was decreased in hypoxia+melatonin group compared with hypoxia group. The experiments have been repeated at least in triplicate. The statistical significance of differences between different groups was calculated using ANOVA. Significant difference between control vs hypoxia groups is shown as *p<0.05 and **p<0.01; significant difference between hypoxia vs hypoxia+melatonin groups is shown as #p<0.05 and ##p<0.01. The values represent the mean ± SD in triplicate. Scale bar = 20 μm.