Figure 2.

The combination therapy represses DNMT isoforms gene expression. CEM and Jurkat cell lines were treated with the single agents and the combination for 48 h followed by RNA extraction and quantitative real time RT-PCR using gene specific primers for the 3 DNMT isoforms DNMT1 (a), DNMT3a (b), and DNMT3b (c). The alphanumeric characters represent the name of the drug and the concentration in nM, where ‘D’ represents DAC and ‘M’ represents DMC. For instance, D100 represents DAC 100 nM and D250 + M500 represents the combination of DAC 250 nM and DMC 500 nM. Data represent the mean ± SD for 3 replicates. d. CEM cells treated with single agents and the combination for 48 h followed by nuclear protein extraction and protein gel blotting.