Figure 5.

The combination of DAC and DMC increases H3K27 acetylation. ChIP analysis of CEM cells treated with single agents or the combination for 24 h was performed using antibodies against H3K36me3 (a) and H3K27Ac (b) followed by real-time quantitative PCR using primers for p15 and CDH-1. IgG was used as a negative control. DAC 250 indicates 250 nM DAC and DMC 500 indicates 500 nM of DMC. D + M indicates the combination of 250 nM DAC and 500 nM DMC. Data represent the mean ± SD of duplicate ChIP reactions and triplicate PCR reactions. * indicates a significant difference from the control at P < 0.05 and^# indicates significant difference from the control and single agents using one way ANOVA followed by Bonferroni post-hoc test.