Fig 4. Organization of the clusters encoding genes involved in toluene degradation and catechol meta-cleavage pathways by P. veronii 1YdBTEX2.

(A) Isopropylbenzene gene cluster (ipb). (B) dmp-like cluster (previously named EXDO A2). (C) nah-like meta-cleavage pathway genes (previously named EXDO A). Genes represented by arrows scaled to size (bp, base-pair) and relevant gene names as well as locus_tag (e.g., PVE_r2g0834) are indicated. Paralogous genes are colored similarly. Note the interruption in the ipbB reading frame by two stop codons (white arrows). Tn, transposable element (black arrows). (D) Proposed metabolic pathway for toluene to acetyl-CoA conversion with relevant chemical structures. Black arrows represent a single enzymatic step with the proposed involved (redundant) gene products indicated below.