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Non-reducing Western blot of apo secretagogin exposed for 2 min (4A) and 5 min (4B) with Pierce™ chemiluminescent Western blotting substrate. Lane 1–11 has 2 μg of 1 mg/mL purified secretagogin with 20 mM DTT, 10 mM DTT, 7 mM DTT, 5 mM DTT, 4 mM DTT, 3 mM DTT, 2 mM DTT, 1 mM DTT, 0.7 mM DTT, 0.5 mM DTT and no DTT respectively. M = monomer; D = dimer; T = trimer.
