Fig 9. Thermal stability of secretagogin as analyzed by circular dichroism spectroscopy when heated from 20 to 95°C in six buffers, A-F (Table 3).

[Θ]_{222 nm} is ellipticity at 222 nm. Blue and pale blue dots represent calcium-loaded secretagogin under heating (20–95°C) and cooling (95–20°C), respectively. Red and pale red dots represent apo secretagogin under heating (20–95°C) and cooling (95–20°C), respectively. A. [Θ]_{222 nm} versus temperature. B. Total absorbance (nm) versus temperature.