Table 3. Various buffer conditions used to study stability of secretagogin.
Label | Buffer | CaCl2 or EDTA | DTT |
---|---|---|---|
A | 10 mM Tris, 0.15 M KCl, pH 7.5 | 1 mM CaCl2 | Absent |
B | 10 mM Tris, 0.15 M KCl, pH 7.5 | 0.5 mM EDTA | Absent |
C | 10 mM Tris, 0.15 M KCl, pH 7.5 | 1 mM CaCl2 | 1 mM |
D | 10 mM Tris, 0.15 M KCl, pH 7.5 | 0.5 mM EDTA | 1 mM |
E | 10 mM Tris, 0.15 M KCl, pH 7.5 | 1 mM CaCl2 | 4 mM |
F | 10 mM Tris, 0.15 M KCl, pH 7.5 | 0.5 mM EDTA | 4 mM |