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. Author manuscript; available in PMC: 2016 Nov 3.
Published in final edited form as: Proteomics Clin Appl. 2014 Aug;8(7-8):590–594. doi: 10.1002/prca.201400029

Figure 1. Ubiquitination of cardiac 20S proteasomes.

Figure 1

(A) The number of identified ubiquitinated lysine in each 20S proteasome subunit is shown. The dashed line represents the spectrum count cutoff for the stringency filter. A modification site is only accepted if identified in ≥ 2 subjects and ≥ 5 independent spectra. (B) The location of the identified lysine ubiquitination sites in 20S proteasome (Protein Data Bank structure 1IRU) alpha and beta subunits. Red-colored residues represent ubiquitination sites; purple residues are ubiquitinated and acetylated. (C) Compared to the aligned sequences around all 20S proteasome lysine positions (left), the ubiquitinated lysines (middle) show a slight enrichment of serine in the +4 position (right). (D) The number of identified acetylated lysine in each 20S proteasome subunit is shown.