Table 1.
Gene expression levels of neuronal marker genes during differentiation of three cell lines.
| SH-SY5Y | LUHMES | NSC | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Marker of Cell: |
Day: | 0 | 1 | 3 | 7 | 0 | 1 | 3 | 7 | 0 | 1 | 3 | 7 |
| N | TUBB3 | 2600 | 2900 | 1100 | 3700 | 2100 | 5600 | 17000 | 22500 | 1400 | 1400 | 2200 | 4600 |
| N | ENO2 | 600 | 800 | 370 | 900 | 21 | 31 | 34 | 1400 | 71 | 57 | 84 | 160 |
| N | MAP2 | 90 | 100 | 46 | 170 | 2 | 10 | 130 | 530 | 300 | 510 | 510 | 590 |
| N | KCNJ6 | 1 | 1 | 1 | 2 | 1 | 1 | 42 | 81 | 15 | 5 | 5 | 7 |
| N | SYN1 | 180 | 250 | 140 | 380 | 1 | 9 | 340 | 630 | 30 | 20 | 31 | 110 |
| N | PINK1 | 95 | 130 | 90 | 200 | 100 | 270 | 240 | 290 | 47 | 60 | 52 | 72 |
| IN | DCX | 480 | 310 | 160 | 260 | 1 | 1 | 92 | 1200 | 36 | 65 | 320 | 920 |
| DN | PARK2 | 20 | 8 | 12 | 21 | 1 | 13 | 11 | 18 | 14 | 10 | 14 | 16 |
| DN | DRD2 | 10 | 15 | 8 | 24 | 1 | 12 | 160 | 350 | 1 | 1 | 3 | 11 |
| DN | TH | 1 | 2 | 1 | 3 | 1 | 1 | 1 | 100 | 1 | 1 | 1 | 1 |
| ON | OLIG2 | 1 | 1 | 1 | 2 | 1 | 1 | 4 | 94 | 2 | 1 | 2 | 5 |
| ON | GALC | 65 | 63 | 28 | 120 | 3 | 9 | 11 | 14 | 1 | 1 | 1 | 1 |
| O | MBP | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 1 |
| A | GFAP | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| A | S100B | 2 | 3 | 2 | 5 | 1 | 1 | 1 | 2 | 8 | 1 | 1 | 1 |
| -- | GAPDH | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 | 10000 |
Genes are listed in the second column in bold and italics. Each gene is a marker for cell types abbreviated in the first column: N, neurons; IN, immature neurons; DN, dopaminergic neurons; ON, oligodendrocytes and neurons; O, oligodendrocytes; G, glia; A, astrocytes. Cell lines and days of differentiation are listed in the first- and second rows. Gene expression values were determined using qPCR. Gene expression values below the limit of detection were set to 1.0 (Ct > 36 were trimmed to Ct = 36 and set to 1). Numbers represent mRNA abundance 2^(36-Normalized Ct(gene)). >Five-fold and >25-fold increases in expression compared to day 0 are highlighted in pink or red, respectively.