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. 2016 May 25;7(24):35577–35591. doi: 10.18632/oncotarget.9590

Figure 5. Increased ROS levels in autophagy-impaired macrophages.

Figure 5

A. ROS staining of Raw264.7 cells with DCF-DA dye revealed that the ROS levels increased in rotenone-treated or bafilomycin A1-treated cells. B. Western blots showing that phosphorylated JNK (p-JNK) and cleaved caspase-1 levels were increased in CoCl2-treated or rotenone-treated Raw264.7 cells and further increased in bafilomycin A1-treated cells and attenuated by rapamycin treatment. C. ROS staining of peritoneal macrophages with DCF-DA dye showing that the ROS level is increased in the Atg7KO mice and further increased by rotenone treatment. D. Western blots showing that the phosphorylation of JNK is elevated in the peritoneal macrophages of Atg7KO mice.