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. 2016 May 25;7(24):35577–35591. doi: 10.18632/oncotarget.9590

Figure 7. Macrophage ROS are required for the regulation of insulin signaling.

Figure 7

A. Peritoneal macrophages from control and Atg7KO mice were treated with rotenone with or without NAC. ROS level measured by DCF-DA staining shows that rotenone-mediated ROS generation is inhibited in peritoneal macrophages from both control and Atg7KO mice. B. Conditioned media were collected from Raw264.7 cells treated with NAC, rotenone, or bafilomycin A1 as indicated and applied to 3T3-L1 cells with or without insulin. Insulin signaling was examined by western blot analysis. C. Conditioned media collected from peritoneal macrophages of control and Atg7KO mice treated with NAC as indicated were applied to 3T3-L1 cells with or without insulin treatment, and insulin signaling was examined by western blot analysis.