Figure 3. Stimulate activity of DC is reduced in the presence of MLN4924.

BALB/c BMDCs were pretreated with or without indicated dose of MLN4924. After isolation and irradiation, CD11c⁺ DCs were used as stimulators of CD4⁺ T cells and co-cultured for A. 4 days and B. 5 days at the ratio of 1:5 and 1:10. The proliferation rate of T cells was reduced with the presence of MLN4924, which means that inhibition of neddylation could decline the stimulate activity of DCs.