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BMDCs were cultured with MLN4924. Total cellular protein extracts were prepared and subjected to immunoblotting by using A. anti-PARP, anti-caspase-3, anti-caspase-7 antibodies and B. anti-cleaved PARP, anti-cleaved-caspase-3, anti-cleaved-caspase-7 antibodies. C. Caspase-3 activity was examined by caspase activity kit. D. Immunoblotting analysis was performed for erk, p-erk, cyclin D1 and cyclin D3 expression.
