Figure 3. Generation of UFLC (Ultra Fast Liquid Chromatography) fingerprints.
San Si formula and all standard samples A., including Loganin, Hyperoside, Specnuezhenide, Quercetin and 5-Hydroxymethylfurfural, as well as immunosuppressive agents Cyclosporin and Rapamycin B. were subject to UFLC analyses using a Shimadzu UFLC system consisting of LC-20AD pump, photodiode-array detector and LC solution chromatographic workstation. One of two independent experiments was shown. The results suggest that the entire San Si formula does not contain traditional immunosuppressive agents cyclosporine and rapamycin.