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. 2016 Apr 7;7(24):35874–35893. doi: 10.18632/oncotarget.8624

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Copyright: © 2016 Huang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Cytotoxic effects of EtOAc extracts of A. marina leaves in AU565, BT483, and HepG2 cancer cells were determined using trypan blue dye exclusion assays (A), DNA fragmentation assays (B), and Hoechst 33258 Dye assays (C) as described in the Materials and Methods. (D) Effects of EtOAc extracts of A. marina leaves on full-length PARP, full-length caspase 8, and cleaved caspase 3 production in AU565, BT483, and HepG2 cancer cells; Cells were treated with various concentrations of EtOAc extracts for 48 h in 2% FBS media. Total protein was collected and western blotting analyses were performed as described in the Materials and Methods. (E) Effects of EtOAc extracts of A. marina leaves on AU565 and BT483 cancer cell viability with or without pretreatment with Z-VAD-FMK caspase inhibitor. Cell viability was determined using MTT assays.

Cytotoxic effects of EtOAc extracts of A. marina leaves in AU565, BT483, and HepG2 cancer cells were determined using trypan blue dye exclusion assays (A), DNA fragmentation assays (B), and Hoechst 33258 Dye assays (C) as described in the Materials and Methods. (D) Effects of EtOAc extracts of A. marina leaves on full-length PARP, full-length caspase 8, and cleaved caspase 3 production in AU565, BT483, and HepG2 cancer cells; Cells were treated with various concentrations of EtOAc extracts for 48 h in 2% FBS media. Total protein was collected and western blotting analyses were performed as described in the Materials and Methods. (E) Effects of EtOAc extracts of A. marina leaves on AU565 and BT483 cancer cell viability with or without pretreatment with Z-VAD-FMK caspase inhibitor. Cell viability was determined using MTT assays.