Figure 4. Sulfasalazine treatment is not gliomatoxic within the brain tumor microenvironment.

Organotypic brain slices (ex vivo) with glioma cell implantation (VOGiM assay) were cultured in the presence of SAS (at 200 μM). A. SAS treatment induces no tumor cell death in glioma-implanted brain slices. F98-GFP expressing glioma cells were implanted in brain slices and the size of the tumor bulk was documented after 5 days by fluorescence imaging. Scale bar represents 1 mm. B. Tumor bulks of the SAS group and the control group were compared quantitatively. There was no significant difference between these two groups. Differences were considered statistically significant with values mean ± SD (*P < 0.05, unpaired two-sided t-test, n ≥ 11 per group).