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. 2016 Apr 29;7(24):36034–36048. doi: 10.18632/oncotarget.9105

Figure 7. The involvement of SMADs in HVJ-E-induced suppression of c-Myc transcription.

Figure 7

(A) Left panel: Time-dependent Smad4 expression was measured in control siRNA- and Smad4 siRNA-transduced NCI-HJ929 cells by immunoblotting analysis. Right panel: Time-dependent effect of HVJ-E on c-Myc mRNA expression in control siRNA- and Smad4 siRNA-transduced NCI-H929 cells by quantitative RT-PCR analysis. The data are presented as the ratio of c-Myc expression compared with baseline c-Myc expression. The data represent the mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, Student's unpaired t-test). (B) Protein levels of phospho-SMAD1/5/8 and c-Myc were analyzed in the MM cell lines NCI-H929 and MM1S with or without HVJ-E treatment (1000 MOI, 8 hours). (C) Measurement of the cytoplasmic and nuclear fraction of activated SMAD1/5/8 in NCI-H929 cells after treatment with HVJ-E with or without BAPTA-AM for 8 hours by immunoblotting.