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. 2016 May 5;7(24):36207–36219. doi: 10.18632/oncotarget.9183

Figure 5. JUN activates lipid accumulation by activating the transcription of srebp1.

Figure 5

(A) Schematic analysis of the potential JUN binding sites in the promoter region of murine srebp1 gene. (B) Results of the luciferase reporter assay conducted in HEK293T cells co-transfected with siRNA specifically inhibiting jun (si- jun-1 or si- jun-2) and a luciferase reporter plasmid containing the promoters of either srebp1 (pGL3-srebp1), srebp1-S1 (pGL3- srebp1-S1) or srebp1-S2 (pGL3- srebp1-S2). (C) A srebp1 ChIP assay was performed in Hep1-6 cells transfected with si- jun/NC (upper panel) or ad- jun/N C (lower panel) for 48 h. (D, E) Western blot analysis of SREBP1 and FAS expression in Hep1-6 and NCTC1469 cells transfected with si-jun-1 or si-jun-2. (F) Oil red O staining of Hep1-6 and NCTC1469 cells transfected with si-jun-1 or si-jun-2. (G) Western blot analysis of SREBP1 and FAS protein levels in Hep1-6 cells co-transfected with si-jun-1 and either the miR-200b inhibitor or the miR-200c inhibitor. The data represent the mean ± SEM of three independent experiments. *P < 0.05 and **P < 0.01 versus the control; &P < 0.05 and &&P < 0.01 versus the miR-200b inhibitor or the miR-200c inhibitor. The bar represents 10 μm.